Cloned (Comment) | Organism |
---|---|
into the HindIII/NotI site of pET-28a(+) plasmid, expression construct is designed to overexpress only L-sorbose reductase protein (residues 1-263), overexpressed in Escherichia coli BL21 (DE3) cells | Gluconobacter frateurii |
Crystallization (Comment) | Organism |
---|---|
in complex with L-sorbose or NADPH using the sitting-drop vapour-diffusion method, at 20°C, to 2.38 and 1.90 A resolution, respectively. Crystal of the L-sorbose reductase-L-sorbose complex belongs to space group C2221, with unit-cell parameters a=124.2, b=124.1, c=60.8 A. The crystal of the L-sorbose reductase-NADPH complex belongs to space group P21, with unit-cell parameters a=124.3, b=61.0, c=124.5A, beta= 89.99°. The crystals contain two and eight molecules, respectively, in the asymmetric unit | Gluconobacter frateurii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter frateurii | A4PB64 | - |
- |
Purification (Comment) | Organism |
---|---|
by sonication, centrifugation and gel filtration | Gluconobacter frateurii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-sorbose + NADPH + H+ | - |
Gluconobacter frateurii | L-sorbitol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-sorbose reductase | - |
Gluconobacter frateurii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Gluconobacter frateurii |